Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.
Fluorescein is compatible with a wide variety of fluorescent instrumentation – most instruments are equipped with FAM channel filters.
This product contains the pure 6-isomer of fluorescein.
General properties
| Appearance: | yellow crystalline solid |
| Mass spec M+ increment: | 358.0 |
| Molecular weight: | 473.39 |
| CAS number: | 92557-81-8 |
| Molecular formula: | C25NO9H15 |
| IUPAC name: | (2,5-dioxopyrrolidin-1-yl) 3′,6′-dihydroxy-1-oxospiro[2-benzofuran-3,9′-xanthene]-5-carboxylate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
| Cat. # | Quantity | Lead time |
| 15120 | 1 mg | 5 days |
| 25120 | 5 mg | in stock |
| 45120 | 25 mg | in stock |
| 55120 | 50 mg | in stock |
| 65120 | 100 mg | in stock |