Lumiprobe TAMRA NHS ester, 5-isomer
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TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore is being used for quite a long time for the preparation of dual labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM).
Just like many other xanthene fluorophores, TAMRA is a available as two isomers (5- and 6-isomer) which have nearly identical optical properties. This product is an isomerically pure 5-TAMRA.
TAMRA NHS is an amine reactive reagent. It can be used for the labeling of proteins, peptides, and modified oligonucleotides containing amine groups.
| Cat. # | Quantity | Lead time |
| 17120 | 1 mg | in stock |
| 27120 | 5 mg | in stock |
| 47120 | 25 mg | in stock |
| 57120 | 50 mg | in stock |
| 67120 | 100 mg | in stock |
General properties
| Appearance: | dark colored solid |
| Molecular weight: | 527.53 |
| CAS number: | 321862-17-3 |
| Molecular formula: | C29H25N3O7 |
| IUPAC name: | (2,5-dioxopyrrolidin-1-yl) 3′,6′-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylate |
| Solubility: | good in DMF, DMSO, low in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
