Lumiprobe BDP R6G NHS ester
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BDP R6G NHS ester is an amine-reactive borondipyrromethene dye (an analog of BODIPY® R6G NHS ester) that has absorption and emission spectra similar to R6G.
BDP R6G is a bright and photostable dye that exhibits long living fluorescence that has little pH dependence. Due to the long fluorescence lifetime, this dye is useful for fluorescence polarization assays, and for two-photon experiments.
The NHS ester function can be conjugated with various amine groups, including those present in proteins and peptides.
General properties
| Appearance: | dark-green crystals |
| Mass spec M+ increment: | 322.1 |
| Molecular weight: | 437.21 |
| CAS number: | 335193-70-9, 1443457-59-7 |
| Molecular formula: | C22H18BF2N3O4 |
| IUPAC name: | 2,5-Dioxo-1-pyrrolidinyl 3-(4,4-difluoro-5-phenyl-3a,4a-diaza-4-bora-s-indacen-3-yl)propionate |
| Solubility: | good in DMF, DMSO, DCM |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 530 |
| Emission maximum, nm: | 548 |
| Fluorescence quantum yield: | 0.96 |
| CF260: | 0.17 |
| CF280: | 0.18 |
