Lumiprobe Coumarin 343 X NHS ester
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Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.
This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.
This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.
| Cat. # | Quantity | Lead time |
| 12620 | 1 mg | in stock |
| 22620 | 5 mg | in stock |
| 42620 | 25 mg | in stock |
| 52620 | 50 mg | in stock |
| 62620 | 100 mg | in stock |
General properties
| Appearance: | yellow solid |
| Molecular weight: | 495.52 |
| CAS number: | 946123-12-2 |
| Molecular formula: | C26H29N3O7 |
| Solubility: | good in DCM, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
