水溶性Cy7-NHS |CAS 477908-53-5|Sulfo-cyanine7 NHS ester
Cy7-N-羟基琥珀酰胺酯
CAS：477908-53-5|
英文名称：Sulfo-cyanine7 NHS ester
产品简介
胺类活性染料，近红外荧光染料，Cy7?的改进的类似物。近红外荧光素可以被用来利用生物组织的近红外窗口。在这个光谱区域，高度增强的组织透明度使得活体成像成为可能。该染料可用于制备CY7标记的生物分子来进行后续试验，如活体研究及药物开发相关实验。Cy7的特殊结构加固了中心聚亚甲基（polymethyne）链的稳定性。这种增强的分子结构会使其量子产率较其母结构提供20%，因此它的荧光强度会更大。
该试剂在标记时需要共溶剂有机溶剂。水溶Cy7 NHS ester可以用于蛋白NIR标记。
Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7®.
NIR fluorophores can be used to take advantage of near infrared window of biological tissues – increased transparency of tissues in this spectral region allows to carry out in vivo imaging.
This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.
The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.
This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.
一般特性
| 外观: | green powder |
| 分子量: | 682.29 |
| 分子式: | C41H48ClN3O4 |
| 溶解性: | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
| 质量控制: | NMR 1H (95%) and 13C, TLC, functional testing |
| 储存条件: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
光谱特性
| 激发波长, nm: | 750 |
| 在激发波长下的消光系数, Lmol-1cm-1: | 199000 |
| 发生波长, nm: | 773 |
| 荧光量子产率: | 0.3 |
| Cat. # | Quantity |
| 15320 | 1 mg |
| 25320 | 5 mg |
| 45320 | 25 mg |
| 55320 | 50 mg |
| 65320 | 100 mg |