Lumiprobe代理 61480 BDP FL maleimide, 100 mg
Thiol reactive BDP FL maleimide is a reactive dye for protein labeling, which has identical structure with BODIPY® FL maleimide.
BDP FL is a borondipyrromethene dye which has absorption and fluorescence spectra similar to fluorescein (FAM). However, this dye exhibits very high photostability. It is non-charged, and has low molecular weight. Its brightness is similar to fluorescein, R110 and xanthene dye derivatives like Alexa Fluor® 488.
This fluorophore is ideal for fluorescent microscopy and many other applications. The fluorophore can substitute fluorescein for almost any application, and it is compatible with any FAM-capable fluorescent instrumentation.
General properties
| Appearance: | orange solid |
| Mass spec M+ increment: | 414.1 |
| Molecular weight: | 414.21 |
| CAS number: | 773859-49-7 |
| Molecular formula: | C20H21BF2N4O3 |
| Solubility: | Good in organic solvents (DMF, DMSO), limited in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 503 |
| ε, L⋅mol−1⋅cm−1: | 92000 |
| Emission maximum, nm: | 509 |
| Fluorescence quantum yield: | 0.97 |
| CF260: | 0.015 |
| CF280: | 0.027 |
| Cat. # | Quantity |
| 11480 | 1 mg |
| 21480 | 5 mg |
| 41480 | 25 mg |
| 51480 | 50 mg |
| 61480 | 100 mg |
Customers also purchased with this product
Sulfo-Cyanine5 NHS ester
Water-soluble Cyanine 5 succinimide ester.
Cyanine5 maleimide
Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.
Cyanine3.5 NHS ester
Cyanine3.5 dye NHS ester derivative.
citations
- Koch, S.; Seinen, A.-B.; Kamel, M.; Kuckla, D.; Monzel, C.; Kedrov, A.; Driessen, A.J.M. Single-molecule analysis of dynamics and interactions: of the SecYEG translocon. FEBS Journal, in press. doi: 10.1111/febs.15596
- São Pedro, M.N.; Azevedo, A.M.; Aires-Barros, M.R.; Soares, R.R.G. Minimizing the influence of fluorescent tags on IgG partition in PEG-salt aqueous two-phase systems for rapid screening applications. Biotechnology Journal, 2019, 14(8), 1800640. doi: 10.1002/biot.201800640
- Doh, J.K.; White, J.D.; Zane, H.K.; Chang, Y.H.; López, C.S.; Enns, C.A.; Beatty, K.E. VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115, 12961–12966. doi: 10.1073/pnas.1808626115
- Buecheler, J.W.; Winzer, M.; Tonillo, J.; Weber, C.A.; Gieseler, H. Impact of Payload Hydrophobicity on Stability of Antibody-Drug-Conjugates. Molecular Pharmaceutics, 2018, 15(7), 2656–2664. doi: 10.1021/acs.molpharmaceut.8b00177