Lumiprobe代理 61480 BDP FL maleimide, 100 mg

Lumiprobe代理 61480 BDP FL maleimide, 100 mg

Thiol reactive BDP FL maleimide is a reactive dye for protein labeling, which has identical structure with BODIPY® FL maleimide.

BDP FL is a borondipyrromethene dye which has absorption and fluorescence spectra similar to fluorescein (FAM). However, this dye exhibits very high photostability. It is non-charged, and has low molecular weight. Its brightness is similar to fluorescein, R110 and xanthene dye derivatives like Alexa Fluor® 488.

This fluorophore is ideal for fluorescent microscopy and many other applications. The fluorophore can substitute fluorescein for almost any application, and it is compatible with any FAM-capable fluorescent instrumentation.

General properties

Appearance: orange solid
Mass spec M+ increment: 414.1
Molecular weight: 414.21
CAS number: 773859-49-7
Molecular formula: C20H21BF2N4O3
Solubility: Good in organic solvents (DMF, DMSO), limited in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 92000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027
Cat. # Quantity
11480 1 mg
21480 5 mg
41480 25 mg
51480 50 mg
61480 100 mg

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citations

  1. Koch, S.; Seinen, A.-B.; Kamel, M.; Kuckla, D.; Monzel, C.; Kedrov, A.; Driessen, A.J.M. Single-molecule analysis of dynamics and interactions: of the SecYEG translocon. FEBS Journal, in press. doi: 10.1111/febs.15596
  2. São Pedro, M.N.; Azevedo, A.M.; Aires-Barros, M.R.; Soares, R.R.G. Minimizing the influence of fluorescent tags on IgG partition in PEG-salt aqueous two-phase systems for rapid screening applications. Biotechnology Journal2019, 14(8), 1800640. doi: 10.1002/biot.201800640
  3. Doh, J.K.; White, J.D.; Zane, H.K.; Chang, Y.H.; López, C.S.; Enns, C.A.; Beatty, K.E. VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy. Proceedings of the National Academy of Sciences of the United States of America2018, 115, 12961–12966. doi: 10.1073/pnas.1808626115
  4. Buecheler, J.W.; Winzer, M.; Tonillo, J.; Weber, C.A.; Gieseler, H. Impact of Payload Hydrophobicity on Stability of Antibody-Drug-Conjugates. Molecular Pharmaceutics2018, 15(7), 2656–2664. doi: 10.1021/acs.molpharmaceut.8b00177