Lumiprobe代理 614E0 BDP FL tetrazine, 100 mg
Inverse electron demand Diels-Alder reaction (ied-DA) with tetrazines is a promising tool for the conjugation of biomolecules. The reaction takes place between tetrazine as an electron acceptor heterodiene, and a strained dienophile, such as trans-cyclooctene, cyclopropene, or some cyclooctynes.
Methyltetrazine provides greater stability in buffers than unsubstituted tetrazine. The rate of its reaction with cycloalkenes still beats almost all other conjugation reaction rates by a factor of magnitudes.
BDP FL is a bright dye for fluorescein (FAM) channel. Using BDP FL tetrazine, the dye can be conjugated with molecules bearing strained olefins.
General properties
| Appearance: | red crystalline solid |
| Mass spec M+ increment: | 447.2 |
| Molecular weight: | 475.3 |
| CAS number: | 2042193-77-9 |
| Molecular formula: | C24H24N7BF2O |
| Solubility: | good in DCM, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 503 |
| ε, L⋅mol−1⋅cm−1: | 92000 |
| Emission maximum, nm: | 509 |
| Fluorescence quantum yield: | 0.97 |
| CF260: | 0.015 |
| CF280: | 0.027 |
114E0 1 mg
214E0 5 mg
414E0 25 mg
514E0 50 mg
614E0 100 mg
Customers also purchased with this product
BDP 558/568 carboxylic acid
BDP 558/568 is a borondipyrromethene dye with yellow emission. The dye as a carboxylic acid can be used in peptide synthesis (after appropriate activation of the carboxylic group).
FAM tetrazine, 6-isomer
This molecule is a conjugate of FAM (fluorescein) – a bright fluorophore, and tetrazine – a reactive group that reacts with trans-cycloalkenes in a reaction called ied-DA (inverse electron demand Diels Alder reaction).
BDP 630/650 azide
BDP 630/650 is a bright borondipyrromethene based fluorophore for the far red area of the spectrum. It possesses high extinction coefficient and quantum yield. This is an azide for Click chemistry labeling.