Lumiprobe代理 614E0 BDP FL tetrazine, 100 mg

Lumiprobe代理 614E0 BDP FL tetrazine, 100 mg

Inverse electron demand Diels-Alder reaction (ied-DA) with tetrazines is a promising tool for the conjugation of biomolecules. The reaction takes place between tetrazine as an electron acceptor heterodiene, and a strained dienophile, such as trans-cyclooctene, cyclopropene, or some cyclooctynes.

Methyltetrazine provides greater stability in buffers than unsubstituted tetrazine. The rate of its reaction with cycloalkenes still beats almost all other conjugation reaction rates by a factor of magnitudes.

BDP FL is a bright dye for fluorescein (FAM) channel. Using BDP FL tetrazine, the dye can be conjugated with molecules bearing strained olefins.

General properties

Appearance: red crystalline solid
Mass spec M+ increment: 447.2
Molecular weight: 475.3
CAS number: 2042193-77-9
Molecular formula: C24H24N7BF2O
Solubility: good in DCM, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.


Spectral properties

Excitation maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 92000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027

114E0 1 mg
214E0 5 mg
414E0 25 mg
514E0 50 mg
614E0 100 mg

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