Lumiprobe代理 42690 Coumarin 343 X carboxylic acid, 25 mg
Coumarin 343 is a blue emitting fluorophore used as a laser dye. The fluorophore can serve as a FRET donor for FAM (fluorescein).
General properties
| Appearance: | yellow crystals |
| Molecular weight: | 398.45 |
| CAS number: | 946123-11-1 |
| Molecular formula: | C22H26N2O5 |
| IUPAC name: | 6-({4-Oxo-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-5-yl}carbonylamino)hexanoic acid |
| Solubility: | Well soluble in DMF, DMSO, moderately soluble in DCM, DCE, EtOAc, insoluble in diethyl ether. |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
| Cat. # | Quantity |
| 12690 | 1 mg |
| 22690 | 5 mg |
| 42690 | 25 mg |
| 52690 | 50 mg |
| 62690 | 100 mg |
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Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.