Lumiprobe代理 62620 Coumarin 343 X NHS ester, 100 mg
Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.
This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.
This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.
General properties
| Appearance: | yellow solid |
| Molecular weight: | 495.52 |
| CAS number: | 946123-12-2 |
| Molecular formula: | C26H29N3O7 |
| Solubility: | good in DCM, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
12620 1 mg
22620 5 mg
42620 25 mg
52620 50 mg
62620 100 mg
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Product citations
- Zheng, L.; Zhao, H.; Han, Y.; Qian, H.; Vukovic, L.; Mecinović, J.; Král, P.; Huck, W.T.S. Catalytic transport of molecular cargo using diffusive binding along a polymer track. Nature Chemistry, 2019, 11(4), 359–366. doi: 10.1038/s41557-018-0204-7