Lumiprobe Cy染料61390 Sulfo-Cyanine3 carboxylic acid, 100 mg

Lumiprobe Cy染料61390 Sulfo-Cyanine3 carboxylic acid, 100 mg

Sulfo-Cyanine3 carboxylic acid

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Water soluble sulfo-Cyanine3 dye, free unactivated monofunctional carboxylic acid. This reagent can be used as a reference fluorophore for Cy3® detection channel, as a control in experiments with other sulfo-Cyanine3 labeled products. Carboxylic acid can be also activated with carbodiimides.

Absorbance and emission spectra are identical with Cy3® fluorophore.

sulfo-Cy3 carboxylic acid fluorophore structure

General properties

Appearance: dark red crystals
Molecular weight: 654.84
CAS number: 1121756-11-3 (inner salt); 1941997-61-0 (sodium salt)
Molecular formula: C30H35N2KO8S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​(5-​carboxypentyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt, potassium salt
Solubility: Well soluble in water, DMF, DMSO (0.55 M = 360 g/L). Practically insoluble in non-polar organic solvents.
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Spectral properties

Excitation maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
Cat. # Quantity
11390 1 mg
21390 5 mg
41390 25 mg
51390 50 mg
61390 100 mg

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Cyanine3 maleimide
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Sulfo-Cyanine5.5 maleimide
Sulfo-Cyanine5.5 is a water-soluble far red emitting dye. This is a thiol reactive maleimide derivative of sulfo-Cyanine5.5. It is recommended for the labeling of sensitive proteins.

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Product citations

  1. Vallmitjana, A.; Torrado, B.; Dvornikov, A.; Ranjit, S.; Gratton, E. Blind Resolution of Lifetime Components in Individual Pixels of Fluorescence Lifetime Images Using the Phasor Approach. Journal of Physical Chemistry B2020, 124(45), 10126–10137. doi: 10.1021/acs.jpcb.0c06946
  2. Cho, U.; Riordan, D.P.; Ciepla, P.; Kocherlakota, K.S.; Chen, J.K.; Harbury, P.B. Ultrasensitive optical imaging with lanthanide lumiphores. Nature Chemical Biology2018, 14(1), 15–21. doi: 10.1038/nchembio.2513
  3. Spears, B.R.; Marin, M.A.; Chaker, A.N.; Lampley, M.W.; Harth, E. Precise Microscale Polymeric Networks through Piezoelectronic Inkjet Printing. ACS Biomaterials Science & Engineering2016, 2(8), 1265–1272. doi: 10.1021/acsbiomaterials.6b00175