Lumiprobe Cyanine5 DBCO 染料
A derivative of Cyanine5 red-emitting fluorophore possessing DBCO (dibenzocyclooctyne, also known as ADIBO, azodibenzocyclooctyne) group for copper free Click chemistry.
Strained cycloalkynes, such as cyclooctynes, react with azides very rapidly in the absence of copper catalyst in a strain promoted alkyne azide cycloaddition (spAAC). This reaction is very fast, mild, and biocompatible.
Compared to other cycloalkynes, DBCO provide among the fastest reaction kinetics, still possessing good stability.
一种具有 DBCO（二苯并环辛炔，也称为 ADIBO，偶氮二苯并环辛炔）基团的 Cyanine5 发红光荧光团的衍生物，用于无铜点击化学。
在不存在铜催化剂的情况下，在应变促进的炔烃叠氮化物环加成 (spAAC) 中，应变的环炔烃，例如环辛炔，与叠氮化物反应非常迅速。 该反应非常快速、温和且具有生物相容性。
与其他环炔烃相比，DBCO 提供了最快的反应动力学，但仍具有良好的稳定性。
| 货号 | 规格 | 货期 | |
|---|---|---|---|
| A30F0 | 1 mg | in stock | |
| B30F0 | 5 mg | in stock | |
| C30F0 | 10 mg | in stock | |
| D30F0 | 25 mg | in stock | |
| E30F0 | 50 mg | in stock | |
| F30F0 | 100 mg | in stock |
General properties
| Appearance: | dark blue solid |
| Mass spec M+ increment: | 928.4 |
| Molecular weight: | 929.03 |
| Molecular formula: | C53H59N4F6O2P |
| Solubility: | good in DMF, DMSO, chlorinated organic solvents, practically insoluble in water (<1 uM, < 1 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Merlo, R.; Caprioglio, D.; Cillo, M.; Valenti, A.; Mattossovich, R.; Morrone, C.; Massarotti, A.; Rossi, F.; Miggiano, R.; Leonardi, A.; Minassi, A.; Perugino, G. The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. Journal of Enzyme Inhibition and Medicinal Chemistry, 2021, 36(1), 85–97. doi: 10.1080/14756366.2020.1841182
- Qiu, C.-X.; Bai, X.-F.; Shen, Y.; Zhou, Z.; Pan, L.-Q.; Xu, Y.-C.; Zhao, W.-B.; Chen, S.-Q. Specific Inhibition of Tumor Growth by T Cell Receptor-Drug Conjugates Targeting Intracellular Cancer-Testis Antigen NY-ESO-1/LAGE-1. Bioconjugate Chemistry, 2020, 31(12), 2767–2778. doi: 10.1021/acs.bioconjchem.0c00548
- Islam, M.R.; Nguy, C.; Pandit, S.; Lyon, L.A. Design and Synthesis of Core–Shell Microgels with One‐Step Clickable Crosslinked Cores and Ultralow Crosslinked Shells. Macromolecular Chemistry and Physics, 2020, 221(19), 2000156. doi: 10.1002/macp.202000156
- Liu, W.; Zhao, W.; Bai, X.; Jin, S.; Li, Y.; Qiu, C.; Pan, L.; Ding, D.; Xu, Y.; Zhou, Z.; Chen, S. High antitumor activity of Sortase A-generated anti-CD20 antibody fragment drug conjugates. European Journal of Pharmaceutical Sciences, 2019, 134, 81–92. doi: 10.1016/j.ejps.2019.04.015