Lumiprobe代理 42630 Coumarin 343 X azide, 25 mg

Lumiprobe代理 42630 Coumarin 343 X azide, 25 mg

Coumarin 343 is a blue emitting fluorophore with an emission maximum around 480 nm. This dye forms a FRET pair with fluorescein, and can harvest blue light energy for the subsequent transfer to other fluorophores.

The azide derivative can be conjugated with alkynes in a copper-catalyzed and copper-free Click chemistry reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye, and the azide function.

Structure of Coumarin 343 X azide

General properties

Appearance: yellow solid
Mass spec M+ increment: 480.3
Molecular weight: 480.56
Molecular formula: C25H32N6O4
IUPAC name: 5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 437
ε, L⋅mol−1⋅cm−1: 39000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63
CF260: 0.29
CF280: 0.24
Cat. # Quantity
12630 1 mg
22630 5 mg
42630 25 mg
52630 50 mg
62630 100 mg

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