LumiPure DNA凝胶提取试剂盒 DNA Gel Extraction Kit

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The kit for rapid and efficient purification of DNA fragments from agarose gels or enzymatic reactions

The LumiPure DNA Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from agarose gels or enzymatic reactions. The DNA fragment of interest is excised from an agarose gel and placed in a microcentrifuge tube. Gel Solubilization Buffer dissolves the agarose gel slice, denatures proteins and ensures selective binding of DNA to the membrane in the column. It also contains a color pH indicator that enables easy control of the optimal pH. If pH shifts toward the alkaline side adding of Neutralization Buffer returns the optimal pH value for DNA binding to the column membrane. In the following step, a series of washings of the column membrane containing DNA is performed. Special formulations of Washing Solutions allow impurities to be completely removed from the column membrane. Finally, the DNA is eluted with Elution Buffer or, if necessary, with deionized water.

Kit Specifications:

• Samples: gel slice or enzymatic reaction containing DNA of interest (from 70 bp to 10 kb in size)
• Operation time: 20 minutes
• Column binding capacity: 5 µg DNA
• Elution Volume: ≥ 10 µL
• Typical DNA recovery: 85–95 %
• DNA Purity: OD₂₆₀/OD₂₈₀ >1.8

The purified DNA is suitable for any subsequent application, such as PCR, restriction enzyme digestion, ligation and transformation, labeling, Southern blotting, preparing samples for Sanger sequencing and NGS, etc.

LumiPure DNA凝胶提取试剂盒用于快速、高效地纯化琼脂糖凝胶或酶促反应中的DNA片段。将感兴趣的DNA片段从琼脂糖凝胶中取出，置于微离心管中。凝胶溶解缓冲液溶解琼脂糖凝胶片、变性蛋白，保证DNA选择性结合到柱上的膜上。它还包含一个彩色pH指示器，可以方便地控制最佳pH值。当pH向碱性侧移动时，中和缓冲液的加入会返回DNA与柱膜结合的最佳pH值。在接下来的步骤中，对含有DNA的柱膜进行一系列清洗。特殊配方的洗涤液允许从柱膜中完全除去杂质。最后，用洗脱缓冲液或必要时用去离子水洗脱DNA。

成套规格：

• 样品：含有感兴趣的DNA的凝胶切片或酶反应(从70 bp到10 kb大小)
• 试验时间：20分钟
• 柱结合容量：5gDNA
• 洗脱体积：≥10升
• 典型DNA回收率：85-95%
• DNA纯度：OD₂₆₀/OD₂₈₀ >1.8

纯化后的DNA可用于PCR、酶切、连接转化、标记、Southern杂交、制备Sanger测序和NGS样品等。

Storage conditions: Store at room temperature.
Storage time in month: 12