Assays that use IRDye 800CW conjugates (such as in vivoimaging and cell binding assays) may require a “dye-only” control for potential effects or retention of the dye.
Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.
Note: The carboxylate dye has no reactive group and cannot be used for labeling.
Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly and was completely cleared after 48 h. Originally published in Kovar, J., et al. (2007) Anal Biochem 367:1-12.
How is IRDye 800CW Carboxylate Used?
In vitro Cell-based Assays
As a control for cell-based assays that monitor binding of a dye-labeled agent.
Validation of optical agents for in vivo administration
Evaluation of binding specificity
In vivo Imaging
For evaluation of behavior and clearance of the dye itself.
Timing of dye clearance from the animal’s body
Retention of dye in certain organs or sites (e.g., liver or kidneys)
Labeling Reaction Reference
As a standard to determine the amount of unreacted (“free”) dye after IRDye 800CW conjugation and purification.
Residual unreacted dye may cause:
Artificially high values when dye/protein (D/P) ratio is calculated
Increased background fluorescence in biological assays
Chemical Formula: C46H50N2Na4O15S4
Molecular Weight: 1091.10 g/mol
Exact Mass: 1090.17
To discuss purchase quantities over 1 gram or commercial development rights using IRDye infrared dyes, please contact Business Development