Note: The carboxylate dye has no reactive group and cannot be used for labeling.

Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly and was completely cleared after 48 h. Originally published in Kovar, J., et al. (2007) Anal Biochem 367:1-12.

Selected P/N:

929-08972,

929-09406,

929-70122,

929-70123,

929-70124,

929-70125,

929-70126

How is IRDye 800CW Carboxylate Used?

In vitro Cell-based Assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

• Validation of optical agents for in vivo administration
• Evaluation of binding specificity

In vivo Imaging

For evaluation of behavior and clearance of the dye itself.

• Timing of dye clearance from the animal’s body
• Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted (“free”) dye after IRDye 800CW conjugation and purification.

Residual unreacted dye may cause:

• Artificially high values when dye/protein (D/P) ratio is calculated
• Increased background fluorescence in biological assays

Dye Structure

Properties

• Chemical Formula: C₄₆H₅₀N₂Na₄O₁₅S₄
• Molecular Weight: 1091.10 g/mol
• Exact Mass: 1090.17