水溶性Cy7-NHS |CAS 477908-53-5|Sulfo-cyanine7 NHS ester

水溶性Cy7-NHS |CAS 477908-53-5|Sulfo-cyanine7 NHS ester

Cy7-N-羟基琥珀酰胺酯

CAS:477908-53-5|

英文名称:Sulfo-cyanine7 NHS ester

产品简介

胺类活性染料,近红外荧光染料,Cy7?的改进的类似物。近红外荧光素可以被用来利用生物组织的近红外窗口。在这个光谱区域,高度增强的组织透明度使得活体成像成为可能。该染料可用于制备CY7标记的生物分子来进行后续试验,如活体研究及药物开发相关实验。Cy7的特殊结构加固了中心聚亚甲基(polymethyne)链的稳定性。这种增强的分子结构会使其量子产率较其母结构提供20%,因此它的荧光强度会更大。

该试剂在标记时需要共溶剂有机溶剂。水溶Cy7 NHS ester可以用于蛋白NIR标记。

Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7®.

NIR fluorophores can be used to take advantage of near infrared window of biological tissues – increased transparency of tissues in this spectral region allows to carry out in vivo imaging.

This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.

The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.

This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.

Sulfo-Cyanine7 NHS ester structure

一般特性

 外观: green powder
分子量: 682.29
分子式: C41H48ClN3O4
溶解性: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility   in water
质量控制: NMR 1H (95%) and 13C, TLC, functional testing
储存条件: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for   up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

光谱特性

激发波长, nm: 750
在激发波长下的消光系数, Lmol-1cm-1: 199000
发生波长, nm: 773
荧光量子产率: 0.3
Cat. # Quantity
15320 1 mg
25320 5 mg
45320 25 mg
55320 50 mg
65320 100 mg

 

Lumiprobe AF488 NHS ester

Lumiprobe AF488 NHS ester

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AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though – otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.

This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Cat. # Quantity Price
11820 1 mg 110.00$
21820 5 mg 290.00$
41820 25 mg 690.00$
51820 50 mg 1270.00$
61820 100 mg 1990.00$

General properties

Appearance: dark orange solid
Molecular weight: 732.74
Molecular formula: C31H32N4O13S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid)
Storage conditions: Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91

 

Lumiprobe BDP 558/568 NHS ester

Lumiprobe BDP 558/568 NHS ester

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BDP 558/568 is a borondipyrromethene fluorophore with emission in the yellow part of the spectrum. Its absorption and emission spectra are in similar range as TAMRA, BDP TMR, Cyanine3, and sulfo-Cyanine3. This is an amine reactive NHS ester.

Chemical structure of BDP 558/568 NHS ester

Cat. # Quantity Lead time
17420 1 mg in stock
27420 5 mg in stock
47420 25 mg in stock
57420 50 mg in stock
67420 100 mg in stock

General properties

Appearance: dark colored solid
Molecular weight: 443.23
Molecular formula: C20H16N3BF2O4S
Solubility: good in DMF, DMSO, ethyl acetate
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 561
ε, L⋅mol−1⋅cm−1: 84400
Emission maximum, nm: 569
CF260: 0.00
CF280: 0.07

Lumiprobe BDP 630/650 X NHS ester

Lumiprobe BDP 630/650 X NHS ester

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BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.

This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.

Structure of BDP 630/650 X NHS ester

Cat. # Quantity Lead time
15420 1 mg in stock
25420 5 mg in stock
45420 25 mg in stock
55420 50 mg in stock
65420 100 mg in stock

General properties

Appearance: dark colored solid
Mass spec M+ increment: 545.2
Molecular weight: 660.5
CAS number: 2213445-35-1; 380367-48-6
Molecular formula: C33H31N4BF2O6S
IUPAC name: Succinimidyl-6-[2-(p-{(E)-2-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]ethenyl}phenoxy)acetylamino]hexanoate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

Lumiprobe BDP 650/665 X NHS ester

Lumiprobe BDP 650/665 X NHS ester

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BDP 650/665 is a borondipyrromethene dye with far red excitation and emission. The dye has a good molar extinction coefficient and emission quantum yield.

The NHS ester can be conjugated with amine groups of proteins, peptides, and other molecules. The molecule contains an additional aminohexanoic acid linker.

BDP 650/665 X NHS ester, 6E420

Structure of BDP 650/665 X NHS ester

Cat. # Quantity Lead time
1E420 1 mg in stock
2E420 5 mg in stock
4E420 25 mg in stock
5E420 50 mg in stock
6E420 100 mg in stock

General properties

Appearance: dark colored solid
Mass spec M+ increment: 528.2
Molecular weight: 643.45
CAS number: 235439-04-0; 1616842-78-4
Molecular formula: C33H32N5BF2O6
Solubility: good in DMF, DMSO, low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 649
ε, L⋅mol−1⋅cm−1: 94000
Emission maximum, nm: 667
Fluorescence quantum yield: 0.52
CF260: 0.04
CF280: 0.04

Lumiprobe BDP R6G NHS ester

Lumiprobe BDP R6G NHS ester

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BDP R6G NHS ester is an amine-reactive borondipyrromethene dye (an analog of BODIPY® R6G NHS ester) that has absorption and emission spectra similar to R6G.

BDP R6G is a bright and photostable dye that exhibits long living fluorescence that has little pH dependence. Due to the long fluorescence lifetime, this dye is useful for fluorescence polarization assays, and for two-photon experiments.

The NHS ester function can be conjugated with various amine groups, including those present in proteins and peptides.

BDP R6G NHS ester

Structure of BDP R6G NHS ester

Cat. # Quantity Lead time
14420 1 mg in stock
24420 5 mg in stock
44420 25 mg in stock
54420 50 mg in stock
64420 100 mg in stock

General properties

Appearance: dark-green crystals
Mass spec M+ increment: 322.1
Molecular weight: 437.21
CAS number: 335193-70-9, 1443457-59-7
Molecular formula: C22H18BF2N3O4
IUPAC name: 2,5-Dioxo-1-pyrrolidinyl 3-(4,4-difluoro-5-phenyl-3a,4a-diaza-4-bora-s-indacen-3-yl)propionate
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 530
Emission maximum, nm: 548
Fluorescence quantum yield: 0.96
CF260: 0.17
CF280: 0.18

Lumiprobe BDP TMR NHS ester

Lumiprobe BDP TMR NHS ester

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BDP TMR is a borondipyrromethene dye, analog of BODIPY® TMR. This is an amine-reactive NHS ester for the labeling of proteins, peptides, and other molecules with amino groups.

BDP TMR is a dye for TAMRA channel, which is however much brighter than TAMRA. Because of rather long life time of excited state, this fluorophore is a good choice for fluorescence anisotropy measurements, especially with the short linker offered. Fluorescence polarization is an excellent method for high throughput binding assays.

Structure of BODIPY TMR NHS ester

Cat. # Quantity Lead time
12420 1 mg in stock
22420 5 mg in stock
42420 25 mg 5 days
52420 50 mg in stock
62420 100 mg in stock

General properties

Appearance: purple solid
Mass spec M+ increment: 380.2
Molecular weight: 495.28
CAS number: 485397-12-4
Molecular formula: C25H24BF2N3O5
Solubility: good in most organic solvents (DMF, DMSO, DCM, acetone, etc.)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 545
Emission maximum, nm: 570
Fluorescence quantum yield: 0.95
CF260: 0.16
CF280: 0.16

 

Lumiprobe 磺酸基CY3.5 NHS酯 sulfo-Cyanine3.5 NHS ester

Lumiprobe 磺酸基CY3.5 NHS酯 sulfo-Cyanine3.5 NHS ester

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Cyanine3.5 is a fluorescent dye with absorption and emission intermediate between Cyanine3 and Cyanine5. Sulfonated version contains four sulfo groups which ensure high solubility and hydrophilicity of the fluorophore and labeled conjugates.

The NHS ester reactive group allows to label primary and secondary amine groups.

sulfo-Cyanine3.5 NHS ester, 62320

Structure of sulfo-Cyanine3.5 NHS ester

Cat. # Quantity Lead time
12320 1 mg in stock
22320 5 mg in stock
42320 25 mg in stock
52320 50 mg in stock
62320 100 mg in stock

General properties

Appearance: deep purple powder
Molecular weight: 1088.33
CAS number: 2231670-91-8 (without cation)
Molecular formula: C42H40N3K3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 576
ε, L⋅mol−1⋅cm−1: 139000
Emission maximum, nm: 603
Fluorescence quantum yield: 0.11
CF260: 0.16
CF280: 0.17

Lumiprobe TAMRA NHS ester, 5-isomer

Lumiprobe TAMRA NHS ester, 5-isomer

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TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore is being used for quite a long time for the preparation of dual labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM).

Just like many other xanthene fluorophores, TAMRA is a available as two isomers (5- and 6-isomer) which have nearly identical optical properties. This product is an isomerically pure 5-TAMRA.

TAMRA NHS is an amine reactive reagent. It can be used for the labeling of proteins, peptides, and modified oligonucleotides containing amine groups.

TAMRA NHS ester, 5-isomer, 67120

Structure of 5-TAMRA NHS ester

Cat. # Quantity Lead time
17120 1 mg in stock
27120 5 mg in stock
47120 25 mg in stock
57120 50 mg in stock
67120 100 mg in stock

General properties

Appearance: dark colored solid
Molecular weight: 527.53
CAS number: 321862-17-3
Molecular formula: C29H25N3O7
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 3′,6′-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylate
Solubility: good in DMF, DMSO, low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19

Lumiprobe 磺酸基CY7.5NHS酯 Sulfo-Cyanine7.5 NHS ester

Lumiprobe 磺酸基CY7.5NHS酯 Sulfo-Cyanine7.5 NHS ester

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Sulfo-Cyanine7.5 is a near infrared water soluble and hydrophilic dye for the NIR imaging applications. The structure and spectra of the dye resemble indocyanine green (ICG) that has been approved for use in humans for years. However, unlike ICG, sulfo-Cyanine7.5 contains a trimethylene bridge that increases its quantum yield compared to ICG, and also has a linker arm for its attachment to proteins, peptides, and other molecules. This derivative is an NHS ester for the modification of amine groups.

Structure of sulfo-Cyanine7.5 NHS ester

Cat. # Quantity Lead time
16320 1 mg in stock
26320 5 mg in stock
46320 25 mg in stock
56320 50 mg in stock
66320 100 mg in stock

General properties

Appearance: dark green solid
Mass spec M+ increment: 950.2
Molecular weight: 1180.47
Molecular formula: C49H48N3K3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 778
ε, L⋅mol−1⋅cm−1: 222000
Emission maximum, nm: 797
CF260: 0.09
CF280: 0.09