Lumiprobe蛋白质组学试剂 Proteomic reagents

Lumiprobe蛋白质组学试剂 Proteomic reagents

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,Lumiprobe上海金畔生物官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品,产品货期10个工作日左右,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规,质量保证,价格优惠。

Lumiprobe蛋白质组学试剂 Proteomic reagents

Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye for protein labeling

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1A041 5 nmol
2A041 10 nmol
3A041 25 nmol

Cyanine3 NHS ester minimal dye

Cyanine3 minimal dye for protein labeling.

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1B041 5 nmol
2B041 10 nmol
3B041 25 nmol

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.

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Cat. #QuantityPriceLead timeBuy this product

1C041 5 nmol
2C041 10 nmol
3C041 25 nmol

产品列表

货号
中文名
产品描述
1A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 5 nmol
2A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 10 nmol
3A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 25 nmol
1B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 5 nmol
2B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 10 nmol
3B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 25 nmol
1C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 5 nmol
2C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 10 nmol
3C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 25 nmol

* 上述中文名称仅供参考,订货以货号和英文名称为准

辅助试剂 Auxiliary reagents
抗坏血酸 Ascorbic acid

ascorbic acid for reduction of Cu(II)-TBTA complex.

Ascorbic acid
铜-TBTA络合物,10mM溶于55%的二甲亚砜水溶液
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

DMF (dimethylformamide)
标记级别二甲基酰胺 DMF (dimethylformamide), labeling grade

Dry high purity amine free solvent for NHS ester labeling.


产品列表

货号
中文名
产品描述
1A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 5 nmol
12050 抗坏血酸 Ascorbic acid, 10 mg
21050 铜-TBTA络合物,10mM溶于55%的二甲亚砜水溶液 Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO, 1.5 mL
13050 标记级别二甲基酰胺 DMF (dimethylformamide), labeling grade, 1 mL

* 上述中文名称仅供参考,订货以货号和英文名称为准

PCR/qPCR PCR/qPCR
罗丹明对照品染料用于快速PCR ROX reference dye for qPCR

ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.

SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x

SYBR Green I stock solution for Real Time PCR, 100x.

SYBR Green I 凝胶染色溶液 SYBR Green I Gel Staining Solution, 10000x

SYBR Green I dye for staining dsDNA in gelen.


产品列表

货号
中文名
产品描述
31110 罗丹明对照品染料用于快速PCR ROX reference dye for qPCR, 500 uL
41010 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 1 mL
51010 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 1.5 mL
51010-5 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 5×1.5 mL
20010 SYBR Green I 凝胶染色溶液 SYBR Green I Gel Staining Solution, 10000x, 1 mL

Lumiprobe点击化学用染料-非荧光炔烃 Non-fluorescent alkynes

Lumiprobe点击化学用染料-非荧光炔烃 Non-fluorescent alkynes

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,上海金畔生物Lumiprobe官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线,所有规格产品保证货期5-7工作日到货,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规(市场上部分是分装,质量不能保证),质量保证,价格极具竞争力,而且我们的货期仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

非荧光炔烃 Non-fluorescent alkynes
生物素-炔烃 Biotin alkyne

Biotin alkyne is a reactive affinity label which can be attached to biomolecules via Click Chemistry.

Biotin alkyne
戊炔酸 STP酯 Pentynoic acid STP ester

An activated ester for the introduction of alkyne into peptides/proteins.

Pentynoic acid STP ester

产品列表

货号
中文名
产品描述
C37B0 生物素-炔烃 Biotin alkyne, 10 mg
D37B0 生物素-炔烃 Biotin alkyne, 25 mg
E37B0 生物素-炔烃 Biotin alkyne, 50 mg
F37B0 生物素-炔烃 Biotin alkyne, 100 mg
30720 戊炔酸 STP酯 Pentynoic acid STP ester, 10 mg
40720 戊炔酸 STP酯 Pentynoic acid STP ester, 25 mg
50720 戊炔酸 STP酯 Pentynoic acid STP ester, 50 mg
60720 戊炔酸 STP酯 Pentynoic acid STP ester, 100 mg

* 上述中文名称仅供参考,订货以货号和英文名称为准

Lumiprobe点击化学用染料 –无荧光叠氮化物 Non-fluorescent azides

Lumiprobe点击化学用染料 –无荧光叠氮化物 Non-fluorescent azides

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,Lumiprobe上海金畔生物官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品,产品货期10个工作日左右,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规,质量保证,价格优惠。

非荧光叠氮化物 Non-fluorescent azides
氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester

Activated ester for azido labeling of peptides and proteins.

Azidobutyric acid NHS ester
生物素-叠氮化物 Biotin azide

Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe.

Biotin azide

产品列表

货号
中文名
产品描述
33720 氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester, 10 mg
43720 氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester, 25 mg
53720 氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester, 50 mg
C3730 生物素-叠氮化物 Biotin azide, 10 mg
D3730 生物素-叠氮化物 Biotin azide, 25 mg
E3730 生物素-叠氮化物 Biotin azide, 50 mg
F3730 生物素-叠氮化物 Biotin azide, 100 mg

* 上述中文名称仅供参考,订货以货号和英文名称为准

Lumiprobe FAM azide, 5-isomer

Lumiprobe FAM azide, 5-isomer

FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.

FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.

Cat. # Quantity
14130 100 uL, 10 mM/DMSO
34130 500 uL, 10 mM/DMSO
44130 1 mL, 10 mM/DMSO
A4130 1 mg
B4130 5 mg
C4130 10 mg
D4130 25 mg
E4130 50 mg
F4130 100 mg

5-FAM azide structure

5-FAM azide

General properties

Appearance: yellowish crystals
Molecular weight: 458.42
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Product citations

  1. Su, H.; Liu, Z.; Liu, Y.; Ma, V.P.-Y.; Blanchard, A.; Zhao, J.; Galior, K.; Dyer, R.B.; Salaita, K. Light-Responsive Polymer Particles as Force Clamps for the Mechanical Unfolding of Target Molecules. Nano Letters, 2018, 18(4), 2630–2636. doi:10.1021/acs.nanolett.8b00459
  2. Long, M.J.C.; Urul, D.A.; Chawla, S.; Lin, H.-Y.; Zhao, Y.; Haegele, J.A.; Wang, Y.; Aye, Y. Precision Electrophile Tagging inCaenorhabditis elegans. Biochemistry, 2018, 57(2), 216–220. doi: 10.1021/acs.biochem.7b00642
  3. Fatona, A.; Berry, R.M.; Brook, M.A.; Moran-Mirabal, J.M. Versatile Surface Modification of Cellulose Fibres and Cellulose Nanocrystals through Modular Triazinyl Chemistry. Chemistry of Materials, 2018, 30(7), 2424–2435. doi: 10.1021/acs.chemmater.8b00511
  4. Pink, M.; Verma, N.; Zerries, A.; Schmitz-Spanke, S. Dose-dependent response to 3-nitrobenzanthrone exposure in human urothelial cancer cells. Chemical Research in Toxicology, 2017, 30(10), 1855–1864. doi: 10.1021/acs.chemrestox.7b00174

Lumiprobe 抗体标记试剂盒sulfo-Cyanine7.5 antibody labeling kit

Lumiprobe 抗体标记试剂盒sulfo-Cyanine7.5 antibody labeling kit

上海金畔生物代理Lumiprobe品牌产品,欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

This is a kit for the labeling of antibodies and proteins of similar size with sulfo-Cyanine7.5 fluorophore. The dye is attached by NHS ester chemistry, and then the excess of the fluorophore is removed by means of gel filtration using enclosed spin columns. The protocol requires only around 40 minutes of time, and provides desalted antibody in PBS buffer containing optimal number of fluorophores per Ab. The kit contains components for 10 reactions, up to 100 ug of antibody per reaction.

这是一种标记抗体和类似大小的蛋白质的试剂盒。染料通过NHS酯化学连接,然后用封闭的自旋柱进行凝胶过滤去除过量的荧光团。该方案只需要40分钟左右的时间,并提供脱盐抗体在PBS缓冲液中含有最佳数量的荧光细胞每抗体。该试剂盒含有10种反应的成分,每次反应的抗体可达100微克。
Cat. # Quantity
6321-10rxn 10 rxn

General properties
Storage conditions: Store at temperature between +3 °C and +20 °C. Do not freeze!

Lumiprobe sulfo-Cyanine5 antibody labeling kit

Lumiprobe sulfo-Cyanine5 antibody labeling kit

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,Lumiprobe上海金畔生物官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品,产品货期10个工作日左右,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规,质量保证,价格优惠。

This is a kit for the labeling of antibodies and proteins of similar size with sulfo-Cyanine5 fluorophore. The dye is attached by NHS ester chemistry, and then the excess of the fluorophore is removed by means of gel filtration using enclosed spin columns. The protocol requires only around 40 minutes of time, and provides desalted antibody in PBS buffer containing optimal number of fluorophores per Ab. The kit contains components for 10 reactions, up to 100 ug of antibody per reaction.

这是一个标记抗体和类似大小的蛋白质与磺胺-氰基-5荧光载体的试剂盒。染料通过NHS酯化学连接,然后用封闭的自旋柱进行凝胶过滤去除过量的荧光团。该方案只需要40分钟左右的时间,并提供脱盐抗体在PBS缓冲液中含有最佳数量的荧光细胞每抗体。该试剂盒含有10种反应的成分,每次反应的抗体可达100微克。
sulfo-Cyanine5 antibody labeling kit
sulfo-Cyanine5 antibody labeling kit
sulfo-Cyanine5 antibody labeling kit
Absorption and emission spectra of sulfo-Cyanine5
Cat. # Quantity
3321-10rxn 10 rxn

General properties
Storage conditions: Store at temperature between +3 °C and +20 °C. Do not freeze!

Lumiprobe sulfo-Cyanine3 antibody labeling kit

Lumiprobe sulfo-Cyanine3 antibody labeling kit

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,Lumiprobe上海金畔生物官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品,产品货期10个工作日左右,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规,质量保证,价格优惠。

Cat. # Quantity
1321-10rxn 10 rxn
A ready to use kit for the labeling of antibodies with sulfo-Cyanine3 dye, a Cy3® equivalent. This kit contains all all necessary components (chemicals and consumables) to perform 10 labelings of antibodies (up to 100 ug antibody per each reaction). Reaction clean up is achieved using enclosed gel filtration spin columns.

Precise quantification of the dye guarantees reproducible outcome of the labeling and number of fluorophores per antibody molecule.

Purified labeled antibodies are available in PBS buffer after 40 min (10 min hands on time). The kit tolerates common additives such as Tris, glycerol, sodium azide.

Photo of Lumiprobe Antibody labeling kit
Photo of Lumiprobe Antibody labeling kit
Photo of Lumiprobe Antibody labeling kit

Absorption and emission spectra of sulfo-Cyanine3

Absorption and emission spectra of sulfo-Cyanine3

General properties
Storage conditions: Store at temperature between +3 °C and +20 °C. Do not freeze! 不要冷冻

Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

Lumiprobe protein labeling kits contain pre-aliquoted reactive dye, all necessary auxiliary components and buffers, and desalting spin columns to achieve efficient, reproducible labeling of antibodies, and other proteins.

The labeled protein is purified by means of gel filtration on gel filled spin columns that are enclosed. This protocol ensures complete removal of the unbound dye, and excellent recovery of the labeled product. The protein is eluted in PBS buffer, ready for the use in most downstream applications.

The protocol is quick, effortless, and easy to follow.

Lumiprobe 探针蛋白标记试剂盒包含预先引用的活性染料、所有必需的辅助成分和缓冲器,以及脱盐自旋柱,以实现抗体和其他蛋白质的高效、可复制的标记。

标记蛋白是通过凝胶过滤纯化的凝胶填充自旋柱是封闭的。该协议确保完全去除未结合染料,并出色地恢复标记产品。该蛋白在PBS缓冲液中被洗脱,可以在大多数下游应用中使用。

该试剂盒具有快速,毫不费力,易于遵循等优点。

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.


sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.


sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.


sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.


sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.