标签归档：Lumiprobe染料

Lumiprobe Cy7.5-N-羟基琥珀酰亚胺酯

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Lumiprobe Cy7.5-N-羟基琥珀酰亚胺酯

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

订购货号 产品名称及规格
16020 Cyanine7.5 NHS ester, 1 mg
26020 Cyanine7.5 NHS ester, 5 mg
46020 Cyanine7.5 NHS ester, 25 mg
56020 Cyanine7.5 NHS ester, 50 mg
66020 Cyanine7.5 NHS ester, 100 mg

Cyanine7.5是一种近红外荧光染料，具有很长的发射光谱，是此类荧光衍生而来的活性胺产品。近红外照射很容易穿透组织因此常应用于体内成像领域。该产品可以用于标记含有氨基的小分子物质，比如蛋白和多肽，通过近红外光谱成像技术（NIR imaging）来示踪他们在有机体内的分布。中心聚亚甲基基团的增强的稳定性使得量子产率较其母结构Cy7.5® NHS提高20%。

推荐手册氨基分子的NHS酯标记

General properties

Appearance: green powder
Mass spec M+ increment: 630.4
Molecular weight: 833.76
CAS number: 1469541-54-5 (without anion), 1469278-04-3 (chloride)
Molecular formula: C49H52N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[2-​[3-​[2-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​ethylidene]​-​1-​cyclohexen-​1-​yl]​ethenyl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

 

Spectral properties

Excitation maximum, nm: 788
Extinction coefficient at excitation maximum, Lmol-1cm-1: 223000
Emission maximum, nm: 808

Product citations

  • Zhang, Z.; Cai, H.; Liu, Z.; Yao, P. Effective Enhancement of Hypoglycemic Effect of Insulin by Liver-targeted Nanoparticles Containing Cholic Acid-modified Chitosan Derivative. Molecular Pharmaceutics, in press. doi:10.1021/acs.molpharmaceut.6b00188
  • Dou, Y.; Guo, J.; Chen, Y.; Han, S.; Xu, X.; Shi, Q.; Jia, Y.; Liu, Y.; Deng, Y.; Wang, R.; Li, X.; Zhang, J. Sustained delivery by a cyclodextrin material-based nanocarrier potentiates antiatherosclerotic activity of rapamycin via selectively inhibiting mTORC1 in mice. Journal of Controlled Release, 2016, 235, 48–62. doi: 10.1016/j.jconrel.2016.05.049
  • Markovic, S.; Belz, J.; Kumar, R.; Cormack, R.A.; Sridhar, S.; Niedre, M. Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants. International Journal of Nanomedicine, 2016, 11, 1213–1223. doi: 10.2147/IJN.S93324
  • Han, F.Y.; Thurecht, K.J.; Lam, A.-L.; Whittaker, A.K.; Smith, M.T. Novel Polymeric Bioerodable Microparticles for Prolonged-Release Intrathecal Delivery of Analgesic Agents for Relief of Intractable Cancer-Related Pain. Journal of Pharmaceutical Sciences, 2015, 104(7), 2334–2344. doi: 10.1002/jps.24497
  • Duong, H.T.T.; Dong, Z.; Su, L; Boyer, C.; George, J.; Davis, T.P.; Wang, J. The Use of Nanoparticles to Deliver Nitric Oxide to Hepatic Stellate Cells for Treating Liver Fibrosis and Portal Hypertension. Small, 2015, 11(19), 2291–2304.201402870
  • Chen, K.; Xu, X.; Guo, J.W.; Zhang, X.; Han, S.; Wang, R.; Li, X.; Zhang, J. Enhanced Intracellular Delivery and Tissue Retention of Nanoparticles by Mussel-Inspired Surface Chemistry. Biomacromolecules, 2015, 16(11), 3574–3583. doi:10.1021/acs.biomac.5b01056
  • Lin, Y.-A.; Cheetham, A.G.; Zhang, P.; Ou, Y.-C.; Li, Y.; Liu, G.; Hermida Merino, D.; Hamley, I.W.; Cui, H. Multi-Walled Nanotubes Formed by Catanionic Mixtures of Drug Amphiphiles. ACS Nano, 2014, 8(12), 12690-12700. doi: 10.1021/nn505688b
  • Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  • Lu, H.D.; Soranno, D.E.; Rodell, C.B.; Kim, I.L.; Burdick, J.A. Secondary Photocrosslinking of Injectable Shear-Thinning Dock-and-Lock Hydrogels. Advanced Healthcare Materials, 2013, 2(7), 1028-1036. doi: 10.1002/adhm.201200343
  • Yang, H.; Mao, H.; Wan, Z.; Zhu, A.; Guo, M.; Li, Y.; Li, X.; Wan, J.; Yang, X.; Shuai, X. et al. Micelles assembled with carbocyanine dyes for theranostic near-infrared fluorescent cancer imaging and photothermal therapy. Biomaterials, 2013, 34(36), 9124-9133. doi: 10.1016/j.biomaterials.2013.08.022

LumiPure DNA凝胶提取试剂盒 DNA Gel Extraction Kit

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LumiPure DNA凝胶提取试剂盒 DNA Gel Extraction Kit

Products /DNA/PCR/qPCR/LumiPure DNA Gel Extraction Kit

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

The kit for rapid and efficient purification of DNA fragments from agarose gels or enzymatic reactions

The LumiPure DNA Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from agarose gels or enzymatic reactions. The DNA fragment of interest is excised from an agarose gel and placed in a microcentrifuge tube. Gel Solubilization Buffer dissolves the agarose gel slice, denatures proteins and ensures selective binding of DNA to the membrane in the column. It also contains a color pH indicator that enables easy control of the optimal pH. If pH shifts toward the alkaline side adding of Neutralization Buffer returns the optimal pH value for DNA binding to the column membrane. In the following step, a series of washings of the column membrane containing DNA is performed. Special formulations of Washing Solutions allow impurities to be completely removed from the column membrane. Finally, the DNA is eluted with Elution Buffer or, if necessary, with deionized water.

Kit Specifications:

  • Samples: gel slice or enzymatic reaction containing DNA of interest (from 70 bp to 10 kb in size)
  • Operation time: 20 minutes
  • Column binding capacity: 5 µg DNA
  • Elution Volume: ≥ 10 µL
  • Typical DNA recovery: 85–95 %
  • DNA Purity: OD260/OD280 >1.8

The purified DNA is suitable for any subsequent application, such as PCR, restriction enzyme digestion, ligation and transformation, labeling, Southern blotting, preparing samples for Sanger sequencing and NGS, etc.

LumiPure DNA凝胶提取试剂盒用于快速、高效地纯化琼脂糖凝胶或酶促反应中的DNA片段。将感兴趣的DNA片段从琼脂糖凝胶中取出，置于微离心管中。凝胶溶解缓冲液溶解琼脂糖凝胶片、变性蛋白，保证DNA选择性结合到柱上的膜上。它还包含一个彩色pH指示器，可以方便地控制最佳pH值。当pH向碱性侧移动时，中和缓冲液的加入会返回DNA与柱膜结合的最佳pH值。在接下来的步骤中，对含有DNA的柱膜进行一系列清洗。特殊配方的洗涤液允许从柱膜中完全除去杂质。最后，用洗脱缓冲液或必要时用去离子水洗脱DNA。

成套规格：

  • 样品：含有感兴趣的DNA的凝胶切片或酶反应(从70 bp到10 kb大小)
  • 试验时间：20分钟
  • 柱结合容量：5gDNA
  • 洗脱体积：≥10升
  • 典型DNA回收率：85-95%
  • DNA纯度：OD260/OD280 >1.8

纯化后的DNA可用于PCR、酶切、连接转化、标记、Southern杂交、制备Sanger测序和NGS样品等。

Cat. # Quantity
23793 50 preps
LumiPure DNA Gel Extraction Kit, 23793
Storage conditions: Store at room temperature.
Storage time in month: 12

Lumiprobe 抗体标记试剂盒sulfo-Cyanine7.5 antibody labeling kit

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Lumiprobe 抗体标记试剂盒sulfo-Cyanine7.5 antibody labeling kit

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

This is a kit for the labeling of antibodies and proteins of similar size with sulfo-Cyanine7.5 fluorophore. The dye is attached by NHS ester chemistry, and then the excess of the fluorophore is removed by means of gel filtration using enclosed spin columns. The protocol requires only around 40 minutes of time, and provides desalted antibody in PBS buffer containing optimal number of fluorophores per Ab. The kit contains components for 10 reactions, up to 100 ug of antibody per reaction.

这是一种标记抗体和类似大小的蛋白质的试剂盒。染料通过NHS酯化学连接，然后用封闭的自旋柱进行凝胶过滤去除过量的荧光团。该方案只需要40分钟左右的时间，并提供脱盐抗体在PBS缓冲液中含有最佳数量的荧光细胞每抗体。该试剂盒含有10种反应的成分，每次反应的抗体可达100微克。
Cat. # Quantity
6321-10rxn 10 rxn

General properties
Storage conditions: Store at temperature between +3 °C and +20 °C. Do not freeze!

Lumiprobe sulfo-Cyanine7 antibody labeling kit

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Lumiprobe sulfo-Cyanine7 antibody labeling kit

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

This is a kit for the labeling of antibodies and proteins of similar size with sulfo-Cyanine7 fluorophore. The dye is attached by NHS ester chemistry, and then the excess of the fluorophore is removed by means of gel filtration using enclosed spin columns. The protocol requires only around 40 minutes of time, and provides desalted antibody in PBS buffer containing optimal number of fluorophores per Ab. The kit contains components for 10 reactions, up to 100 ug of antibody per reaction.

Cat. # Quantity
5321-10rxn 10 rxn

General properties
Storage conditions: Store at temperature between +3 °C and +20 °C. Do not freeze!

Lumiprobe sulfo-Cyanine5.5 antibody labeling kit

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Lumiprobe sulfo-Cyanine5.5 antibody labeling kit
上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

This is a kit for the labeling of antibodies and proteins of similar size with sulfo-Cyanine5.5 fluorophore. The dye is attached by NHS ester chemistry, and then the excess of the fluorophore is removed by means of gel filtration using enclosed spin columns. The protocol requires only around 40 minutes of time, and provides desalted antibody in PBS buffer containing optimal number of fluorophores per Ab. The kit contains components for 10 reactions, up to 100 ug of antibody per reaction.

General properties
Storage conditions: Store at temperature between +3 °C and +20 °C. Do not freeze!

Cat. # Quantity
7321-10rxn 10 rxn

References

  1. Si, Y.; Guenter, R.; Ou, J.; Kim, S.; Ernst, P.; Carter, A.M.; Bibb, J.A.; Markert, J.M.; Jaskula-Sztul, R.; Zhou, L.; Chen, H.; Liu, X. Novel anti-somatostatin receptor 2 antibody-drug conjugate for neuroendocrine cancer therapy. bioRxiv, preprint. doi: 10.1101/688184

Lumiprobe sulfo-Cyanine3 antibody labeling kit

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Lumiprobe sulfo-Cyanine3 antibody labeling kit

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，Lumiprobe上海金畔生物官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品，产品货期10个工作日左右，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规，质量保证，价格优惠。

Cat. # Quantity
1321-10rxn 10 rxn
A ready to use kit for the labeling of antibodies with sulfo-Cyanine3 dye, a Cy3® equivalent. This kit contains all all necessary components (chemicals and consumables) to perform 10 labelings of antibodies (up to 100 ug antibody per each reaction). Reaction clean up is achieved using enclosed gel filtration spin columns.

Precise quantification of the dye guarantees reproducible outcome of the labeling and number of fluorophores per antibody molecule.

Purified labeled antibodies are available in PBS buffer after 40 min (10 min hands on time). The kit tolerates common additives such as Tris, glycerol, sodium azide.

Photo of Lumiprobe Antibody labeling kit
Photo of Lumiprobe Antibody labeling kit
Photo of Lumiprobe Antibody labeling kit

Absorption and emission spectra of sulfo-Cyanine3

Absorption and emission spectra of sulfo-Cyanine3

General properties
Storage conditions: Store at temperature between +3 °C and +20 °C. Do not freeze! 不要冷冻

Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

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Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

Lumiprobe protein labeling kits contain pre-aliquoted reactive dye, all necessary auxiliary components and buffers, and desalting spin columns to achieve efficient, reproducible labeling of antibodies, and other proteins.

The labeled protein is purified by means of gel filtration on gel filled spin columns that are enclosed. This protocol ensures complete removal of the unbound dye, and excellent recovery of the labeled product. The protein is eluted in PBS buffer, ready for the use in most downstream applications.

The protocol is quick, effortless, and easy to follow.

Lumiprobe 探针蛋白标记试剂盒包含预先引用的活性染料、所有必需的辅助成分和缓冲器，以及脱盐自旋柱，以实现抗体和其他蛋白质的高效、可复制的标记。

标记蛋白是通过凝胶过滤纯化的凝胶填充自旋柱是封闭的。该协议确保完全去除未结合染料，并出色地恢复标记产品。该蛋白在PBS缓冲液中被洗脱，可以在大多数下游应用中使用。

该试剂盒具有快速，毫不费力，易于遵循等优点。

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.

sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.

sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.

sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.

sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.

Lumiprobe 磺酸基CY5 NHS染料 Sulfo-Cyanine5 bis-NHS ester

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Lumiprobe 磺酸基CY5 NHS染料 Sulfo-Cyanine5 bis-NHS ester

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

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Sulfo-Cyanine5 bis-NHS ester is a water soluble bifunctional dye possessing two NHS ester functions. It is an analog of Cy5® bis-NHS ester.

The dye is useful for the cross-conjugation of amines, and for other advanced labeling applications.

Sulfo-Cyanine5 bis-NHS ester, 633D0

Structure of Sulfo-Cyanine5 bis-NHS ester

Cat. # Quantity Lead time
133D0 1 mg in stock
233D0 5 mg in stock
433D0 25 mg in stock
533D0 50 mg in stock
633D0 100 mg in stock

General properties

Appearance: dark blue powder
Molecular weight: 975.14
Molecular formula: C45H51KN4O14S2
Solubility: good in DMF, DMSO, water
Quality control: NMR 1H, HPLC-MS (895%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04