分类目录归档：Lumiprobe N-羟基琥珀酰亚胺酯染料 Dye NHS esters

Lumiprobe BDP TR X NHS ester

发表于由

Lumiprobe BDP TR X NHS ester

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

BDP TR is a borondipyrromethene dye which has absorption and emission wavelengths close to Texas Red (ROX) dye. The fluorophore is bright, moderately hydrophobic, and has low molecular weight. An extra linker based on aminocaproic acid (C6) provide better separation between the dye, and the biomolecule.

The NHS ester function can be used for the conjugation with proteins, peptides, and other molecules possessing amine group.

Structure of BDP TR X NHS ester

Cat. # Quantity Lead time
1D420 1 mg in stock
2D420 5 mg in stock
4D420 25 mg in stock
5D420 50 mg in stock
6D420 100 mg in stock

General properties

Appearance: dark colored solid
Mass spec M+ increment: 519.2
Molecular weight: 634.46
Molecular formula: C31H29N4BF2O6S
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 589
ε, L⋅mol−1⋅cm−1: 60000
Emission maximum, nm: 616
Fluorescence quantum yield: 0.9
CF260: 0.15
CF280: 0.19

Lumiprobe ROX NHS ester, 6- isomer

发表于由

Lumiprobe ROX NHS ester, pure 6- isomer

ROX(rhodamine X aka R101)是一种高荧光强度的、耐光的罗丹明染料，在多领域有广泛的应用。ROX标记的寡核苷酸探针常用于qPCR和有ROX通道的qPCR仪器。
该活性染料用于标记多肽、蛋白、氨基寡核苷酸的氨基基团。Pure single isomer.
ROX NHS ester可以替代Alexa Fluor 568。

ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel.

This is reactive dye for the labeling of amino-groups in peptides, proteins, and amino-oligonucleotides. Pure single isomer.

This dye can replace Alexa Fluor 568, Texas Red.

Absorption and emission spectra of ROX dye

订购货号 产品名称及规格
12220 ROX NHS ester, pure 6- isomer, 1 mg
22220 ROX NHS ester, pure 6- isomer, 5 mg
42220 ROX NHS ester, pure 6- isomer, 25 mg
52220 ROX NHS ester, pure 6- isomer, 50 mg
62220 ROX NHS ester, pure 6- isomer, 100 mg

General properties

Appearance: dark crimson powder
Mass spec M+ increment: 516.2
Molecular weight: 631.67
Molecular formula: C37H33N3O7
CAS number: 117491-83-5
Solubility: good in polar organic solvents (DMF, DMSO), low in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 570
Extinction coefficient at excitation maximum, Lmol-1cm-1: 82000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.0

Lumiprobe Cy3.5 NHS ester 氨基反应性荧光染料,不溶于水

发表于由

Lumiprobe Cy3.5 NHS ester 氨基反应性荧光染料,不溶于水

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，Lumiprobe上海金畔生物官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品，产品货期10个工作日左右，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规，质量保证，价格优惠。

服务热线:18301939375   QQ：3258089810    3259632176

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, Alexa Fluor 594, DyLight 594.

订购货号 产品名称及规格
12020 Cyanine3.5 NHS ester, 1 mg
22020 Cyanine3.5 NHS ester, 5 mg
42020 Cyanine3.5 NHS ester, 25 mg
52020 Cyanine3.5 NHS ester, 50 mg
62020 Cyanine3.5 NHS ester, 100 mg

General properties

Appearance: dark purple powder
Molecular weight: 690.27
Molecular formula: C42H44ClN3O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 591
Extinction coefficient at excitation maximum, Lmol-1cm-1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35

Product citations

  • Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
  • Massey, M.; Kim, H.; Conroy, E.M.; Algar, W.R. Expanded Quantum Dot-Based Concentric Förster Resonance Energy Transfer: Adding and Characterizing Energy-Transfer Pathways for Triply Multiplexed Biosensing. The Journal of Physical Chemistry C, 2017, 121(24), 13345–13356. doi: 10.1021/acs.jpcc.7b02739
  • Wang, C.; Wang, Y.; Li, Y.; Bodemann, B.; Zhao, T.; Ma, X.; Huang, G.; Hu, Z.; DeBerardinis, R.J.; White, M.A.; Gao, J. A nanobuffer reporter library for fine-scale imaging and perturbation of endocytic organelles. Nature Communications, 2015, 6, 8524. doi:10.1038/ncomms9524
  • Fu, B.; Flynn, J.D.; Isaacoff, B.P.; Rowland, D.J.; Biteen, J.S. Super-Resolving the Distance-Dependent Plasmon-Enhanced Fluorescence of Single Dye and Fluorescent Protein Molecules. The Journal of Physical Chemistry C, 2015, 119(33), 19350–19358. doi: 10.1021/acs.jpcc.5b05154

 

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester

发表于由

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester

水溶Cyanine 5琥珀酰亚胺酯（succinimidyl ester（SE）），类似于Cy5® NHS ester，不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。Sulfo-Cyanine 5是Cy5®的衍生物，与很多仪器如读板机（plate readers），显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。
该染料是高度亲水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester。可以在所有的应用中替代Cy5®，Alexa Fluor 647和DyLight 649。

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester   一般特性

外观: dark blue powder
分子量: 761.84
分子式: C36H40N3NaO10S2
溶解性: very good in water, good in DMF and DMSO
质量控制: NMR 1H (95%) and 13C, TLC, functional testing
储存条件: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

光谱特性

最大激发波长, nm: 646
在最大激发波长下的消光系数, Lmol-1cm-1: 271000
最大发生波长, nm: 662
荧光量子产率: 0.2
CF260: 0.13
CF280: 0.13

Cy5 NHS ester structure

Sulfo-Cyanine5 NHS ester

上海金畔生物科技有限公司还可以提供其他相关产品：

Cyanine3 alkyne

Cy3 alkyne

Cyanine3 azide

Cy3 azide

Cy3 N3

Cyanine3 carboxylic acid  1144107-76-5

Cy3 carboxylic acid

Cyanine3 hydrazide

Cyanine3 maleimide

Cy3 maleimide

Cyanine3 NHS ester   1032678-38-8

Cy3 NHS ester

Cyanine3.5 azide

Cyanine3.5 carboxylic acid  1144107-79-8

Cyanine3.5 NHS ester

Cy3.5 NHS ester

Cyanine5 alkyne

Cyanine5 amine

Cy5 amine

Cy5 NH2

Cyanine5 azide

Cy5 N3

Cyanine5 Boc-hydrazide

Cyanine5 carboxylic acid

Cyanine5 hydrazide

Cyanine5 maleimide

Cy5 maleimide

Cyanine5 NHS ester  1032678-42-4

Cy5 NHSester

Cyanine5.5 alkyne

Cyanine5.5 azide

Cyanine5.5 carboxylic acid  1144107-80-1

Cyanine5.5 maleimide

Cyanine5.5 NHS ester

Cyanine7 amine   1650635-41-8

Cy7 amine

Cy7 NH2

Cyanine7 azide

Cy7 azide

Cyanine7 carboxylic acid

Cyanine7 maleimide

Cy7 maleimide

Cyanine7 NHS ester

Cy7 NHS ester

Cyanine7.5 amine

Cyanine7.5 azide

Cyanine7.5 carboxylic acid   1686147-68-1

Cyanine7.5 maleimide

Cyanine7.5 NHS ester

Sulfo-Cyanine 5 NHS ester

Sulfo-Cy 5 NHS ester

Sulfo-Cyanine3 azide

Sulfo-Cy3 azide

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 maleimide

Sulfo-Cy3 maleimide

Sulfo-Cyanine3 NHS ester

Sulfo-Cy3 NHS ester

Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 azide

Sulfo-Cy5 azide

Sulfo-Cyanine5 carboxylic acid   1121756-16-8

Sulfo-Cyanine5 maleimide

Sulfo-Cy5 maleimide

Sulfo-Cyanine7 azide

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 NHS ester

Sulfo-Cy7 NHS ester

 

 

Lumiprobe BDP 581/591 NHS ester

发表于由

Lumiprobe BDP 581/591 NHS ester

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

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BDP 581/591 is a borondipyrromethene dye that has relatively long fluorescence lifetime and two photon excitation cross section. It is useful for fluorescence polarization assays. Due to the presence of a diene system, it can react with reactive oxygen species (ROS) with a change of fluorescence.

This is the NHS ester derivative for the conjugation with primary and secondary amine groups of proteins, peptides, and other molecules.

BDP 581/591 NHS ester

Structure of BDP 581/591 NHS ester

Cat. # Quantity Price Lead time
16420 1 mg 110.00$ in stock
26420 5 mg 210.00$ in stock
46420 25 mg 410.00$ in stock
56420 50 mg 695.00$ in stock
66420 100 mg 1190.00$ in stock

General properties

Appearance: dark colored solid
Mass spec M+ increment: 374.1
Molecular weight: 489.28
Molecular formula: C26H22N3BF2O4
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 585
ε, L⋅mol−1⋅cm−1: 104000
Emission maximum, nm: 594
Fluorescence quantum yield: 0.83
CF260: 0.06
CF280: 0.04

Lumiprobe Coumarin 343 X NHS ester

发表于由

Lumiprobe Coumarin 343 X NHS ester

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.

This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.

This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.

Coumarin 343 X NHS ester, 12620

Structure of Coumarin 343 X NHS ester

Cat. # Quantity Lead time
12620 1 mg in stock
22620 5 mg in stock
42620 25 mg in stock
52620 50 mg in stock
62620 100 mg in stock

General properties

Appearance: yellow solid
Molecular weight: 495.52
CAS number: 946123-12-2
Molecular formula: C26H29N3O7
Solubility: good in DCM, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 437
ε, L⋅mol−1⋅cm−1: 39000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63
CF260: 0.29
CF280: 0.24

Lumiprobe-Cyanine3.5 NHS ester

发表于由
Lumiprobe-Cyanine3.5 NHS ester

Cyanine3.5 NHS ester（Cy3.5® NHS ester的类似物）是标记多肽、蛋白的氨基基团和寡核苷酸的活性染料。可以替代Cy3.5®, Alexa Fluor 594, DyLight 594。

一般性质
外观：深紫色粉末
分子量：690.27
分子式：C42H44ClN3O4
溶解性：溶于有机溶剂（DMF, DMSO,二氯甲烷），不溶于水。
储存条件：储存：收到货后于-20℃黑暗保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。
光谱性质
最大激发波长：591
在最大激发波长下的消光系数：116000
最大发射波长：604
荧光量子产率：0.35

Cy® is a trademark of GE Healthcare.

General properties

 

Appearance: dark purple powder
Molecular weight: 690.27
Molecular formula: C42H44ClN3O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 591
Extinction coefficient at excitation maximum, Lmol-1cm-1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35

 

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, Alexa Fluor 594, DyLight 594.

Cyanine3.5 NHS ester
Lumiprobe-Cyanine3.5 NHS ester结构式：
Cy3.5 NHS ester structure
订购货号 产品名称及规格
12020 Cyanine3.5 NHS ester, 1 mg
22020 Cyanine3.5 NHS ester, 5 mg
42020 Cyanine3.5 NHS ester, 25 mg
52020 Cyanine3.5 NHS ester, 50 mg
62020 Cyanine3.5 NHS ester, 100 mg

Lumiprobe Cyanine5.5 NHS ester

发表于由

Lumiprobe Cyanine5.5 NHS ester

Cy5.5 NHS是近红外胺类活性染料。

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.

CY5.5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。CY5.5属于是近红外荧光染料，当背景荧光有干扰时适宜用它来做标记以进行荧光分析。它也可以用于活体成像实验。该染料可以替代Alexa Fluor 680和DyLight 680。

General properties
Appearance: dark blue solid
Molecular weight: 767.66
CAS number: 1469277-96-0, 2054318-82-8 (chloride); 1469541-53-4, 1874250-60-8 (without anion)
Molecular formula: C44H46N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[5-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03
订购货号 产品名称及规格
17020 Cyanine5.5 NHS ester, 1 mg
27020 Cyanine5.5 NHS ester, 5 mg
47020 Cyanine5.5 NHS ester, 25 mg
57020 Cyanine5.5 NHS ester, 50 mg
67020 Cyanine5.5 NHS ester, 100 mg

Product citations

  1. Liu, L.; Xu, Y.; Zhang, P.; You, J.; Li, W.; Chen, Y.; Li, R.; Rui, B.; Dou, H. High-order Assembly Towards Polysaccharide-Based Complex Coacervate Nanodroplets Capable of Targeting Cancer Cells. Langmuir, 2020, 36(29), 8580–8588. doi: 10.1021/acs.langmuir.0c01458
  2. Lim, Y.G.; Kim, H.J.; Kim, J.W.; Park, K. Calcium-binding near-infrared fluorescent nanoprobe for bone tissue imaging. Journal of Industrial and Engineering Chemistry, 2020, 89, 442–447. doi: 10.1016/j.jiec.2020.06.018
  3. Martín-Moreno, A.; Jiménez Blanco, J.L.; Mosher, J.; Swanson, D.R.; García Fernández, J.M.; and Sharma, A.; Ceña, V.; Muñoz-Fernández, M.A. Nanoparticle-Delivered HIV Peptides to Dendritic Cells a Promising Approach to Generate a Therapeutic Vaccine. Pharmaceutics, 2020, 12(7), 656. doi: 10.3390/pharmaceutics12070656
  4. Srinageshwar, B.; Florendo, M.; Clark, B.; Johnson, K.; Munro, N.; Peruzzaro, S.; Antcliff, A.; Andrews, M.; Figacz, A.; Swanson, D.; Dunbar, G.L.; Sharma, A.; Rossignol, J. A Mixed-Surface Polyamidoamine Dendrimer for In Vitro and In Vivo Delivery of Large Plasmids. Pharmaceutics, 2020, 12(7), 619. doi: 10.3390/pharmaceutics12070619

Lumiprobe Cy5 NHS 酯 Cyanine5 NHS ester

发表于由
Lumiprobe Cy5 NHS 酯 Cyanine5 NHS ester

上海金畔生物代理Lumiprobe品牌产品，欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

During the last years, Cyanine5 (an analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.

Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist – Cyanine5 NHS ester can replace activated esters of Cy5®, Alexa Fluor 647, and DyLight 649.

在过去的一些年，Cy5荧光基因是生命科学研究和诊断领域非常受欢迎的标记试剂。在红色区域有最大的荧光发射，许多CCD检测器有最大灵敏性，并且背景低。染料颜色非常深，因此即使在凝胶上少至1nmol的量用肉眼也可观察到。
Cy5 NHS酯是用来标记多肽，蛋白和寡核苷酸氨基的活性染料。此染料只需少量的共溶剂（比如DMF或DMSO）来标记反应。此试剂用来高效标记可溶性蛋白，各种多肽和寡核苷酸的氨基。对于较复杂的靶标比如易降解的蛋白，不能用DMF或DMSO时，可以考虑用水溶性sulfo-Cyanine 5 NHS 酯不需要共溶剂，并且两者有非常相似的荧光特性。
Cy5荧光基因与各种设备兼容包括许多荧光显微镜，成像仪，扫描设备和荧光成像仪等。
   Cy5 NHS酯可以替代Alexa Fluor 647, and DyLight 649和Cy3.5®活性酯。
分子结构
一般性质
外观：深蓝色粉末
分子量：616.19
分子式：C36H42ClN3O4
CAS号：1032678-42-4, 350686-88-3
溶解性：易溶于有机溶剂（DMSO,DMF和氯化物），水中的溶解性低。
质控：NMR 1H（95%）和13C，TLC, 功能测试
储存条件：储存：收到货后于-20℃避光保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。

General properties

Appearance: dark blue powder
Molecular weight: 616.19
CAS number: 1032678-42-4, 350686-88-3
Molecular formula: C36H42ClN3O4
Solubility: good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents, low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

光谱性质：
最大激发波长：646
在最大激发波长下的消光系数：250000
最大发射波长：662
荧光量子产率：0.2

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Cat. # Quantity Lead time
13020 1 mg in stock
23020 5 mg in stock
43020 25 mg in stock
53020 50 mg in stock
63020 100 mg in stock
Product citations  参考文献
  1. Li, L.; Sun, W.; Li, L.; Liu, Y.; Wu, L.; Wang, F.; Zhou, Z.; Zhang, Z.; Huang, Y. A pH-responsive sequential-disassembly nanohybrid for mitochondrial targeting. Nanoscale, in press. doi: 10.1039/c6nr07004c
  2. Matsuo, Y.; Maurer, S.P.; Yukawa, M.; Zakian, S.; Singleton, M.R.; Surrey, T.; Toda, T. An unconventional interaction between Dis1/TOG and Mal3/EB1 promotes the fidelity of chromosome segregation. Journal of Cell Science. doi: 10.1242/jcs.197533
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Lumiprobe Cy7染料 Cyanine7 NHS ester

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Lumiprobe Cy7染料 Cyanine7 NHS ester

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Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7®.

NIR fluorophores can be used to take advantage of near infrared window of biological tissues – increased transparency of tissues in this spectral region allows to carry out in vivo imaging.

This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.

The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.

This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.

胺类活性染料，近红外荧光染料，Cy7®的改进的类似物。

近红外荧光素可以被用来利用生物组织的近红外窗口。在这个光谱区域，高度增强的组织透明度使得活体成像成为可能。该染料可用于制备CY7标记的生物分子来进行后续试验，如活体研究及药物开发相关实验。

Cy7的特殊结构加固了中心聚亚甲基（polymethyne）链的稳定性。这种增强的分子结构会使其量子产率较其母结构提供20%，因此它的荧光强度会更大。
该试剂在标记时需要共溶剂有机溶剂。水溶Cy7 NHS ester可以用于蛋白NIR标记。

General properties

Appearance: dark green solid
Molecular weight: 733.64
CAS number: 1432019-64-1 (chloride), 1432466-81-3 (without anion)
Molecular formula: C41H48N3BF4O4
IUPAC name: 1‐{6‐[(2,5‐dioxopyrrolidin‐1‐yl)oxy]‐6‐oxohexyl}‐ 3,3‐dimethyl‐2‐[(E)‐2‐[(3E)‐3‐{2‐[(2E)‐1,3,3‐ trimethyl‐2,3‐dihydro‐1H‐indol‐2‐ ylidene]ethylidene}cyclohex‐1‐en‐1‐yl]ethenyl]‐3H‐ indol‐1‐ium tetrafluoroborate
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 199000
Emission maximum, nm: 773
Fluorescence quantum yield: 0.3
CF260: 0.022
CF280: 0.029
Lumiprobe-Cyanine7 NHS ester结构式：

订购货号 产品名称及规格
15020 Cyanine7 NHS ester, 1 mg
25020 Cyanine7 NHS ester, 5 mg
45020 Cyanine7 NHS ester, 25 mg
55020 Cyanine7 NHS ester, 50 mg
65020 Cyanine7 NHS ester, 100 mg